IPSC-DYR0100-CAS9 cell line

EN CN
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location： Home > Products > Cas9 Stable Cell Lines > IPSC-DYR0100-CAS9

EZ-editor™ Gene-editing Series Products

   CRISPR-iScreen™ Library

CRISPR-iScreen™ Library Plasmid

CRISPR-iScreen™ Library Virus

CRISPR-iScreen™ Cell Pool

   gRNA Plasmid Bank

   Cell Lines

KO Cell Lines

Wild-type Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cas9 Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Luciferase Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

EGFP Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Stem Cell Lines

   Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

   Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

   Lentivirus

gene KO/PM cell lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

IPSC-DYR0100-CAS9 Order

IPSC-DYR0100-CAS9 cell line
Catalog#
YC-C098-Cas9-H
Size
1*10^6
Transfection Culture Medium
EZ-Stem™ Cell Culture Medium
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
IPSC-DYR0100-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
IPSC-DYR0100-CAS9
Cell Morphology
Spherical clone
Passage ratio
1:6-1:8
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
EZ-Stem™ Cell Culture Medium
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the IPSC-DYR0100 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a IPSC-DYR0100-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
IPSC-DYR0100-CAS9 Cas9 21.07497406 7.93462086
IPSC-DYR0100-CAS9 β-actin 13.14035320
IPSC-DYR0100 Cas9 34.49109268 21.41424656
IPSC-DYR0100 β-actin 13.07684612
Cutting Efficiency Validation
IPSC-DYR0100-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the IPSC-DYR0100-CAS9 stable cell pool which is electroporated by ABCA gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:IPSC-DYR0100		 STR Info (Cell bank)
Cell Line:ATCC- DYR0100 hiPSC
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11131113
D7S820 910910
D16S539 12121212
VWA 14191419
TH01 9.39.39.39.3
AMEL XYXY
TPOX 811811
CSF1PO 711711
D12S391 1819.3
FGA 2122
D2S1338 1719
D21S11 3032.2
D18S51 1013
D8S1179 1415
D3S1358 1517
D6S1043 1213
PENTAE 77
D19S433 1415
PENTAD 1213
D1S1656 1217.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
IPSC-DYR0100-CAS9 cell line
IPSC-DYR0100-CAS9
Catalog#
YC-C098-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

KO Cell Lines

  • ARHGEF18 Knockout cell line (IPSC)

    ARHGEF18 Knockout cell line (IPSC)
      inquiry   More details

    Organism: Human
    Gene Name: ARHGEF18
    Gene ID: 23370
    Size: 1*10^6
    Catalog#: YKO-H985
    YKO-H985(IPSC-DYR0100)
  • SVEP1 Knockout cell line (Vips)

    SVEP1 Knockout cell line (Vips)
      inquiry   More details

    Organism: Human
    Gene Name: SVEP1
    Gene ID: 79987
    Size: 1*10^6
    Catalog#: YKO-H984
    YKO-H984(IPSC-DYR0100)
  • NR1H2 Knockout cell line (Vips)

    NR1H2 Knockout cell line (Vips)
      inquiry   More details

    Organism: Human
    Gene Name: NR1H2
    Gene ID: 7376
    Size: 1*10^6
    Catalog#: YKO-H879
    YKO-H879(IPSC-DYR0100)

EGFP Stable Cell Lines

Stem Cell Lines

Related Popular Products

KO Cell Lines

  • ARHGEF18 Knockout cell line (IPSC)

    ARHGEF18 Knockout cell line (IPSC)

    Organism: Human
    Gene Name: ARHGEF18
    Gene ID: 23370
    Size: 1*10^6
    Catalog#: YKO-H985
      inquiry   More details
  • SVEP1 Knockout cell line (Vips)

    SVEP1 Knockout cell line (Vips)

    Organism: Human
    Gene Name: SVEP1
    Gene ID: 79987
    Size: 1*10^6
    Catalog#: YKO-H984
      inquiry   More details
  • NR1H2 Knockout cell line (Vips)

    NR1H2 Knockout cell line (Vips)

    Organism: Human
    Gene Name: NR1H2
    Gene ID: 7376
    Size: 1*10^6
    Catalog#: YKO-H879
      inquiry   More details

EGFP Stable Cell Lines

Stem Cell Lines

  • IPSC-DYR0100 cell line

    Human induced pluripotent stem cells (iPSC)(IPSC-DYR0100)

    Price (USD): -
    Catalog#: YC-C098
    Size: 1*10^6
    Instruction: Human induced pluripotent stem cells (iPSC)(IPSC-DYR0100)
      inquiry   More details

WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
Contact us